Journal: JCI Insight
Article Title: The MUC5B promoter variant results in proteomic changes in the nonfibrotic lung
doi: 10.1172/jci.insight.189636
Figure Lengend Snippet: ( A and B ) Volcano plots comparing the MUC5B promoter variant and WT allele in nonfibrotic control ( A ) small airways and ( B ) alveoli, showing the negative natural log of adjusted P values (0.05) for each protein. ( C ) Representative images for the IHC against phosphorylated STAT5A at Tyr694 on nonfibrotic control lungs. Scale bars: 50 μm. ( D ) Quantification of C showing the percentage of positive cells ( n = 12, balanced for MUC5B; 5 fields of view per patient with a total of 22,999 counted nuclei). ** P < 0.005 by unpaired, 2-tailed t test.
Article Snippet: Antibodies against the following proteins were used: p-eIF2α (Cell Signaling Technology, 3398S; 1:50 in EDTA), cytokeratin 17 (Abcam, ab53707; 1:16,000 in HIER), ATF4 (Proteintech, 10835-1-AP; 1:16,000 in HIER), ATF6 (Abcam, ab227830; 1:1,500 in HIER), MUC5B (Novus Bio, NBP2-50522; 1:8,000 in HIER), p-STAT5A Tyr694 (Cell Signaling Technology, 9359S; 1:500 in HIER), CD9 (Abcam, ab2215; 1:80,000 in HIER), and MYOF (Invitrogen, PA5-53134; 1:8,000 in HIER).
Techniques: Variant Assay, Control